Journal: International Journal of Biological Sciences

Article Title: ELTD1 promotes invasion and metastasis by activating MMP2 in colorectal cancer

doi: 10.7150/ijbs.62293

Figure Lengend Snippet: Upregulation of ELTD1 expression in colon tumors from AOM/DSS-treated mice. (A) Establishment of the AOM/DSS-induced CAC model. (B, C) Representative photographs of macroscopic and histological observations (H&E) in colon tissue. (D) qRT-PCR measurement of ALDH1A3, ELTD1, EYA4, MYOD, and TERT expression in tumor tissues from AOM/DSS-treated mice or colon tissues from negative control mice. The results are shown as the mean ± SD (n = 10 for each group), **p<0.01 and *p<0.05. Scale bar=100 μm.

Article Snippet: Tissue slides were sequentially incubated with polyclonal rabbit anti-ELTD1 antibodies (Genetex, USA) at a 1:1000 dilution at 4°C overnight.

Techniques: Expressing, Quantitative RT-PCR, Negative Control

Journal: International Journal of Biological Sciences

Article Title: ELTD1 promotes invasion and metastasis by activating MMP2 in colorectal cancer

doi: 10.7150/ijbs.62293

Figure Lengend Snippet: ELTD1 was highly expressed in CRC and predicted poor prognosis. (A) Relative ELTD1 expression levels in CRC tissues and adjacent normal CRC tissues as detected by qRT-PCR (n=86). (B) Representative images of IHC staining of ELTD1 in CRC tissues and adjacent normal tissues. (C) Survival probability curves based on the expression of ELTD1 in CRC patients (n=279). *p<0.05. Scale bar=100 μm.

Article Snippet: Tissue slides were sequentially incubated with polyclonal rabbit anti-ELTD1 antibodies (Genetex, USA) at a 1:1000 dilution at 4°C overnight.

Techniques: Expressing, Quantitative RT-PCR, Immunohistochemistry

Journal: International Journal of Biological Sciences

Article Title: ELTD1 promotes invasion and metastasis by activating MMP2 in colorectal cancer

doi: 10.7150/ijbs.62293

Figure Lengend Snippet: Relationship between ELTD1 expression and clinicopathologic features of CRC patients (n = 86)

Article Snippet: Tissue slides were sequentially incubated with polyclonal rabbit anti-ELTD1 antibodies (Genetex, USA) at a 1:1000 dilution at 4°C overnight.

Techniques: Expressing

Journal: International Journal of Biological Sciences

Article Title: ELTD1 promotes invasion and metastasis by activating MMP2 in colorectal cancer

doi: 10.7150/ijbs.62293

Figure Lengend Snippet: Knockdown of ELTD1 inhibited CRC cell migration and invasion. (A, B) The efficiency of ELTD1 siRNA in HT29, RKO and HCT116 cells was verified by qRT-PCR (A) and Western blotting (B) . (C) Representative DAPI staining and statistical results of the migration of ELTD1-silenced CRC cells in the Transwell assay. (D) Invasion abilities of the two CRC cell lines were assessed by Transwell assay, followed by crystal violet staining and statistical analysis. The results are shown as the mean ± standard deviation (SD) of three independent experiments. **p <0.01 and *p<0.05. Scale bar=100 μm.

Article Snippet: Tissue slides were sequentially incubated with polyclonal rabbit anti-ELTD1 antibodies (Genetex, USA) at a 1:1000 dilution at 4°C overnight.

Techniques: Knockdown, Migration, Quantitative RT-PCR, Western Blot, Staining, Transwell Assay, Standard Deviation

Journal: International Journal of Biological Sciences

Article Title: ELTD1 promotes invasion and metastasis by activating MMP2 in colorectal cancer

doi: 10.7150/ijbs.62293

Figure Lengend Snippet: Overexpression of ELTD1 promoted CRC cell migration and invasion. (A, B) The mRNA and protein expression levels of ELTD1 were measured by qRT-PCR and Western blotting, respectively after transfection with the pEnCMV-ELTD1-3xFLAG plasmid. β-actin was used as the internal control. (C) Representative DAPI staining and statistical results of CRC cells in the Transwell assay. (D) Representative crystal violet staining and statistical results of CRC cells in the invasion assay. The results are shown as the mean ± standard deviation (SD) of three independent experiments. **p<0.01 and *p<0.05. Scale bar=100 μm.

Article Snippet: Tissue slides were sequentially incubated with polyclonal rabbit anti-ELTD1 antibodies (Genetex, USA) at a 1:1000 dilution at 4°C overnight.

Techniques: Over Expression, Migration, Expressing, Quantitative RT-PCR, Western Blot, Transfection, Plasmid Preparation, Control, Staining, Transwell Assay, Invasion Assay, Standard Deviation

Journal: International Journal of Biological Sciences

Article Title: ELTD1 promotes invasion and metastasis by activating MMP2 in colorectal cancer

doi: 10.7150/ijbs.62293

Figure Lengend Snippet: ELTD1 promoted CRC cell metastasis in vivo. (A) Representative gross photographs and in vivo fluorescence images of lung metastasis of mice treated with ELTD1 or vector as indicated. (n=5 for each group) (B) Representative HE-stained lung tissue samples. (C) Statistical results of metastasis nodule weight from the ELTD1 overexpression and control groups. Scale bar=100 μm.

Article Snippet: Tissue slides were sequentially incubated with polyclonal rabbit anti-ELTD1 antibodies (Genetex, USA) at a 1:1000 dilution at 4°C overnight.

Techniques: In Vivo, Fluorescence, Plasmid Preparation, Staining, Over Expression, Control

Journal: International Journal of Biological Sciences

Article Title: ELTD1 promotes invasion and metastasis by activating MMP2 in colorectal cancer

doi: 10.7150/ijbs.62293

Figure Lengend Snippet: ELTD1 regulated the expression of MMP2 at the transcriptional level. (A) mRNA expression levels of invasiveness-related genes in CRC cells were measured after ELTD1 knockdown. (B) Transcriptional activity of the MMP2 promoter was assessed after silencing ELTD1 expression in HT29, RKO and HCT116 cells. The results are shown as the mean ± standard deviation (SD) of three independent experiments. **p<0.01, *p<0.05

Article Snippet: Tissue slides were sequentially incubated with polyclonal rabbit anti-ELTD1 antibodies (Genetex, USA) at a 1:1000 dilution at 4°C overnight.

Techniques: Expressing, Knockdown, Activity Assay, Standard Deviation

Journal: International Journal of Biological Sciences

Article Title: ELTD1 promotes invasion and metastasis by activating MMP2 in colorectal cancer

doi: 10.7150/ijbs.62293

Figure Lengend Snippet: ELTD1 actively regulated the migration and invasion of CRC cells through MMP2. (A, B) The efficiency of the cotransfection of ELTD1 siRNA and MMP2-expressing plasmids into HT29, RKO and HCT116 cells was verified by qRT-PCR and Western blotting. (C, D) Transwell migration and invasion results of HT29 and RKO cells cotransfected with the MMP2 expression vector and si-ELTD1. The results are shown as the mean ± standard deviation (SD) of three independent experiments. **p<0.01, *p<0.05, ## p<0.01 and # p<0.05. Scale bar=100 μm.

Article Snippet: Tissue slides were sequentially incubated with polyclonal rabbit anti-ELTD1 antibodies (Genetex, USA) at a 1:1000 dilution at 4°C overnight.

Techniques: Migration, Cotransfection, Expressing, Quantitative RT-PCR, Western Blot, Plasmid Preparation, Standard Deviation

Journal: BMC cancer

Article Title: Tumor endothelial ELTD1 as a predictive marker for treatment of renal cancer patients with sunitinib.

doi: 10.1186/s12885-020-06770-z

Figure Lengend Snippet: Fig. 2 Representative immunohistochemical images of ELTD1, CD34 and VEGFR2. Images demonstrating low ELTD1 (a), CD34 (b), VEGFR2 (c) and high ELTD1 (d), CD34 (e) and VEGFR2 (f) staining in the primary renal tumor vasculature in patients later treated for metastatic disease with sunitinib in the first or second line setting

Article Snippet: In this study we used the primary rabbit polyclonal antibody towards ELTD1 (HPA025229, Atlas Antibodies, Stockholm, Sweden), CD34 (CAB000018, Dako Cat#7165, Agilent (Formerly DakoCytomation) and VEGFR2 (CAB004028, Cell Signaling Technology Cat#2479, Cell Singaling Technology, Inc).

Techniques: Immunohistochemical staining, Staining

Journal: BMC cancer

Article Title: Tumor endothelial ELTD1 as a predictive marker for treatment of renal cancer patients with sunitinib.

doi: 10.1186/s12885-020-06770-z

Figure Lengend Snippet: Fig. 3 a and b: Progression free survival (3A) and overall survival (3B) and expression of ELTD1 in sunitinib treated patients. Patients treated for metastatic renal cell cancer with sunitinib in the first or second line setting (n = 77) comparing ELTD1 low versus ELTD1 high. c and d: Progression free survival (3C) and overall survival (3D) in sorafenib treated and expression of ELTD1. Patients treated for metastatic renal cell cancer with sorafenib in the first or second line setting (n = 53) comparing ELTD1 low versus ELTD1 high

Article Snippet: In this study we used the primary rabbit polyclonal antibody towards ELTD1 (HPA025229, Atlas Antibodies, Stockholm, Sweden), CD34 (CAB000018, Dako Cat#7165, Agilent (Formerly DakoCytomation) and VEGFR2 (CAB004028, Cell Signaling Technology Cat#2479, Cell Singaling Technology, Inc).

Techniques: Expressing